The Louis W. Bassett Nature Preserve is 98.1 hectares (242.1 acres) in size and protects various forest stand types as well as wet cedar forest areas, which could hold rare/uncommon species.

The Speer Hillside Nature Preserve is 26.8 hectares (66.4 acres) in size and contains many ecologically significant features including species associated with mixed-age Appalachian hardwood forest, as well as a wetland providing riparian habitat.

The Bliss Island Nature Preserve is 42.1 hectares (103.9 acres) in size and is an ecologically diverse area, which provides habitat to many species, including the North Atlantic right whale (Eubalaena glacialis) and harbour porpoise (Phocoena phocoena).

The Big Rock Nature Preserve is 64.4 hectares (164.1 acres) in size and is comprised of different hardwood, softwood, and mixed wood stand types. Steep slopes on the preserve contain large, mature trees and rich wet areas and streams are located at the base of steep slopes in the north-east portion of the preserve.

The Nicol Family Nature Preserve is 6.8 hectares (16.8 acres) in size and consists mostly of grassy wetlands. The wetland and the river flood seasonally in the freshet. This preserve protects an important Species at Risk in New Brunswick and it forms part of an Ecologically Significant Area featuring extensive waterfowl breeding habitat.

The Keiko & Errol Nature Preserve on Ross Island protects 372 hectares (919.2 acres) of rocky shores, rich coastal forest and wetland and is located next to Grand Manan island, in the beautiful Bay of Fundy. This island is classified as an Ecologically Significant Area and is part of the Grand Manan Archipelago Important Bird Area. The Keiko & Errol Nature Preserve on Ross Island contains critical habitat which provides refuge for many rare and uncommon plant and wildlife species.

The Minister’s Face Nature Preserve extension consists of 8.4 hectares (20.8 acres), bringing the amount of protected land on Long Island to 173.3 hectares (427.5 acres). The cliffs of Minister’s Face have been recognized as an Environmentally Significant Area due to the presence of habitat for rare arctic flora and the spectacular view from the eastern banks of the Kennebecasis.

The Frye Island Nature Preserve extension consists of 215.3 hectares (532 acres) protecting the remainder of the island. This brings the amount of protected land to 369.9 hectares (942.26 acres). This preserve encompasses one large island and several smaller islands, all connecting at low tide. Frye Island is designated as an Environmentally Significant Area for migratory raptors and other birds. The nutrient-rich upwellings caused by the incredibly high tides in the Bay of Fundy attract many birds, terrestrial mammals, and marine life.